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彭云丽，代迎欢，何琴，周旭春Vol.39(3):-
摘要：观察感染后肠易激综合征小鼠肠组织中IL-17、occludin和ZO-1的动态改变。40只C57BL/6小鼠随机分为对照组、感染组（感染后2、4、6、8周组）。感染组小鼠用0.2mL含～条旋毛虫的生理盐水灌胃。于感染后2、4、6、8周，记录小鼠体质量，检测腹壁撤退反射（AWR），连续8h收集小鼠粪便，计算粪便含水百分数。HE染色观察肠道病理改变，免疫组织化学法及Westernblotting检测回盲部和结肠中IL-17、occludin和ZO-1的表达。感染后第2周小鼠肠道出现急性炎症反应，体质量显著降低（P=0.）；至第8周肠道炎症和小鼠体质量基本恢复至正常。结直肠扩张容量为0.35和0.5mL时，感染组AWR评分均高于对照组（P0.01）。感染组粪便含水百分数均高于对照组（P0.05）。免疫组化及Westernblotting结果显示：与对照组相比，感染后2周组IL-17降低（P0.05），感染后8周组IL-17增高（P0.05）；感染组occludin和ZO-1均低于对照组（P0.05）。IL-17的动态改变及紧密连接蛋白occludin和ZO-1的减少可能是导致PI-IBS小鼠内脏高敏感性和粪便含水百分数增高的原因之一，参与了PI-IBS的发生。
关键词：感染后肠易激综合征,回盲部,结肠,IL-17,occludin,ZO-1,动态改变
Abstract：：ToobservethedynamicchangesofintestinalIL-17，occludin，andZO-1inmicewithpost-infectiousirritablebowelsyndrome（PI-IBS）.FortyC57BL/6micewererandomlydividedinto5groups：controlgroupandinfectiongroups（2weeks，4weeks，6weeks，and8weeksaftertrichinellainfection）.Infectiongroupsweregivenbygavagingof~Trichinellaspiralisin0.2mLofnormalsaline.Thebodyweightofmicewererecordedatweek2，4，6，and8afterinfection.Thevisceralsensitivityofmicewasmeasuredbytheabdominalwithdrawalreflex（AWR）.Thestoolwascollectedcontinuouslyfor8hourstocalculatethepercentageoffecalwatercontent.PathologicalchangesofgutwereobservedbyHEstaining.TheexpressionsofIL-17，occludin，andZO-1inileocecusandcolonweredetectedbyimmunohistochemistryandWesternblotting.Atweek2afterinfection，theacuteinflammationoftheintestinaltractwasobservedandthebodyweightofmiceweresignificantlydecreased（P=0.）.Untilweek8afterinfection，theintestinalinflammationandbodyweightofmicerecoveredtonormal.Whenthecolorectaldilatationcapacitywas0.35and0.5mL，theAWRscoresintheinfectiongroupsweresignificantlyhigherthanthoseinthecontrolgroup（P0.01）.Thepercentagesoffecalwatercontentintheinfectiongroupsweresignificantlyhigherthanthoseinthecontrolgroup（P0.05）.Comparedwiththecontrolgroup，theexpressionsofIL-17weresignificantlydecreasedinweek2group（P0.05）andincreasedinweek8group（P0.05）.TheexpressionsofoccludinandZO-1intheinfectiongroupsweresignificantlylowerthanthoseinthecontrolgroup（P0.05）.ThedynamicchangesofIL-17andthedecreaseofTightjunctionproteinsmaybeoneofthemechanismsofvisceralhypersensitivityandincreasedper?centagesoffecalwatercontent.TheymaybeinvolvedinthedevelopmentofPI-IBS.
Keywords：post-infectiousirritablebowelsyndrome ileocecus colon IL-17 occludin ZO-1 dynamicchanges
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