QuantitativeProfilingofGutMicrobiotaofChildrenwithDiarrhea-PredominantIrritableBowelSyndrome

TheAmericanJournalofGastroenterology()

doi:10./ajg..

Abstract

OBJECTIVES:Humanintestinalmicrobiotahasanumberofimportantrolesinhumanhealthandisalsoimplicatedinseveralgastrointestinaldisorders.Thegoalofthisstudywastodeterminethegutmicrobiotaintwogroupsofpre-andadolescentchildren:healthyvolunteersandchildrendiagnosedwithdiarrhea-predominantirritablebowelsyndrome(IBS-D).

METHODS:PhylogeneticMicrobiotaArraywasusedtoobtainquantitativemeasurementsofbacterialpresenceandabundanceinsubjects’fecalsamples.Weutilizedhigh-throughputDNAsequencing,quantitativePCR,andfluorescentinsituhybridizationtoconfirmmicroarrayfindings.

RESULTS:BothsamplegroupsweredominatedbythephylaFirmicutes,Bacteroidetes,andActinobacteria,whichcumulativelyconstituted91%ofoverallsample







































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